{"id":585,"date":"2026-08-26T21:17:21","date_gmt":"2026-08-26T18:17:21","guid":{"rendered":"https:\/\/en.iepor.site\/?page_id=585"},"modified":"2026-08-27T15:28:08","modified_gmt":"2026-08-27T12:28:08","slug":"laboratory-of-molecular-mechanisms-of-cell-transformation","status":"publish","type":"page","link":"https:\/\/en.iepor.site\/?page_id=585","title":{"rendered":"Laboratory of Molecular Mechanisms of Cell Transformation"},"content":{"rendered":"\n<div class=\"lab-head\">\n<div class=\"lh-role\">Head<\/div>\n<div class=\"lh-name\">Elena Kashuba<\/div>\n<div class=\"lh-deg\">Dr Sci (Biol.), Professor<\/div>\n<hr class=\"lh-line\">\n<div class=\"lh-contacts\">\n<span class=\"lh-tel\">+38 (044) 259-01-88<\/span>\n<span class=\"lh-mail\"><a href=\"mailto:Kashuba@nas.gov.ua\">Kashuba@nas.gov.ua<\/a>, <a href=\"mailto:lenakash@yahoo.com\">lenakash@yahoo.com<\/a><\/span>\n<\/div>\n<\/div>\n\n\n\n<h3 class=\"lab-h3\">Priority areas of research<\/h3>\n\n<ul class=\"lab-list\">\n  <li>Our laboratory&#x27;s research is carried out within the framework of the Institute&#x27;s scientific directions aimed at identifying markers of malignant cell transformation and signs of stemness in tumor cells. The obtained results will be the background for development of panels of markers for early diagnosis and prognosis of the course of the cancer disease.<\/li>\n  <li>Our research includes<\/li>\n  <li>identification of molecular mechanisms of malignant cell transformation in order to create new diagnostic and\/or prognostic markers of carcinogenesis;<\/li>\n  <li>study on the properties and a role of MRPS18 family proteins in cancerogenesis and in the control of cell stemness and differentiation;<\/li>\n  <li>search for the causes of blocking activation of B-cells at chronic lymphocytic leukemia (CLL) conditions<\/li>\n<\/ul>\n\n<h3 class=\"lab-h3\">Keywords<\/h3>\n\n<ul class=\"lab-list\">\n  <li>malignant cell transformation, mitochondrial ribosomal proteins of the S18 family (MRPS18), MRPS18-2, Epstein-Barr virus, lymphomas, childhood tumors, embryonic tumors of the central nervous system, stem cells, differentiation, stemness, retinoblastoma-associated protein RB, B-cell chronic lymphocytic leukemia (CLL)<\/li>\n<\/ul>\n\n<button class=\"accordion-blog\">Research<\/button>\n<div class=\"panel-blog\">\n<ul class=\"lab-list\">\n  <li>1.1. Current priority research, modern methods<\/li>\n  <li>The scientific interest of the laboratory is to identify molecular mechanisms of cell transformation to search for new diagnostic and\/or prognostic markers of carcinogenesis.<\/li>\n  <li>Particular attention is paid to studying the properties and role of MRPS18 family proteins in the control of cell stemness, differentiation and carcinogenesis, as well as to clarifying the functional consequences of the interaction of MRPS18-2 and RB proteins in the control of cell stemness and in the carcinogenesis of brain tumors, especially in cases of childhood tumors.<\/li>\n  <li>One of the directions of the laboratory&#x27;s research is to establish the reasons for the blocking of activation, proliferation and apoptosis in B cells in chronic lymphocytic leukemia (CLL).<\/li>\n  <li>1.2. The most significant results for 2021-2025<\/li>\n  <li>In vitro experiments showed that the proteins of the MRPS18 family have different biochemical properties, namely &#8212; the half-life of the MRPS18-1 protein is 90 min., and MRPS18-3 is degraded in a few minutes; MRPS18-2 is practically not destroyed. The data obtained indicate a possible function of the MRPS18-2 protein in maintaining the euchromatin configuration, which allows the active expression of a number of genes controlling cell proliferation.<\/li>\n  <li>A possible promoter region of the MRPS18-2 gene was identified by bioinformatic analysis, where three binding sites for the transcription factor KLF4 protein and one for KLF6 are located. The interaction of the transcription factor KLF4 with the promoter region of the MRPS18-2 gene was confirmed by chromatin immunoprecipitation, and the dependence of the activation of the transcription of the MRPS18-2 gene on the presence of KLF4 was shown by quantitative PCR. KLF4 and KLF6 proteins are important for maintaining the functional characteristics of stem cells and also play an important role in malignant transformation and progression of tumors of various genesis. The dependence of the activation of the transcription of the MRPS18-2 gene on the presence of KLF4, which induces cell pluripotency, partly explains the high level of MRPS18-2 expression in stem and tumor cells.<\/li>\n  <li>We have previously shown that primary fibroblasts in which MRPS18-2 is overexpressed acquire stem cell properties and differentiate into osteoblasts, chondrocytes and adipocytes in vitro. We have now shown that WERI retinoblastoma tumor cells overexpressing MRPS18-2 differentiate into osteoblasts and chondrocytes. Thus, the role of MRPS18-2 in the control of functional plasticity of cells is universal, making this protein a target for antitumor therapy.<\/li>\n  <li>We found that the mRNA and protein expression patterns of RB1 in brain tumors were largely unidirectional. Heterogeneity was found in the expression pattern of RB1 in astrocytoma, glioblastoma, and atypical meningioma samples, with 20 to 60% of tumors being positive, while all embryonal tumors of the central nervous system showed RB1 expression at the mRNA and protein levels.<\/li>\n  <li>The MRPS18-1-3 genes showed different expression patterns depending on the type of brain tumor. The highest mRNA expression of MRPS18-1 was found in glioblastoma samples in both tumor samples and peripheral blood. Overall, MRPS18-1 expression was higher in G4 tumors compared to G2. MRPS18-3 was expressed at higher levels in G2 and embryonal tumors. MRPS18-2 was expressed in all studied samples, regardless of type. The MRPS18-2 protein signal was detected at high levels in most brain tumors.<\/li>\n  <li>Assessment of the level of MRPS18-1 gene expression in peripheral blood can be used as a potential prognostic marker of tumor progression.<\/li>\n  <li>We have established that B-cells of patients with chronic lymphocytic leukemia (CLL) are characterized by a significant decrease in the expression of a number of key transcription factors critical for normal B-cell differentiation. In particular, reduced expression of PU.1, EBF1, Spi-B, BLIMP1, Bcl6, IRF4 and IRF8 was noted, which indicates a dysregulation of several stages of B-lymphocyte development &#8212; from early stages to terminal plasma differentiation. At the same time, the level of IL1 expression remained stable, and IL6 was practically absent. At the same time, a decrease in IL17 expression at the mRNA level was detected compared to conditionally normal B cells. A complete absence of STAT3 and STAT5B expression, a significant decrease in STAT5A and high heterogeneity of STAT6 expression were also recorded.<\/li>\n  <li>The detected changes indicate a systemic disruption of cytokine signaling pathways, which further enhances the failure of normal B-cell differentiation in CLL, which demonstrate an immature phenotype. A decrease in the activity of these transcription factors and STAT proteins may contribute to the formation of a microenvironment that supports the survival of tumor cells and their resistance to BCR signals. This, in turn, enhances the adaptability of leukemic cells and may be one of the factors of their aggressiveness and therapeutic resistance.<\/li>\n  <li>1.3. Additional scientific research areas (if any)<\/li>\n  <li>Cervical cancer is the second most common cancer in women in the EU, which is associated with human papillomavirus (HPV) infection. The development of such malignancies can be prevented by HPV screening and HPV vaccination. Genotyping of HPV associated with the development of cervical cancer is currently being conducted, which will have diagnostic\/prognostic value.<\/li>\n  <li>Effect of irradiation (in the terahertz range) on the viability of human cells. Effect of bacterial lysates on the growth of human cells.<\/li>\n  <li>Delivery of proteins, peptides, and oligonucleotides into mammalian cells on the surface of gold nanoparticles.<\/li>\n  <li>Modern methods<\/li>\n  <li>In our work the methods of molecular biology (isolation of genomic DNA, RNA, cDNA synthesis, quantitative PCR, study of RNA degradation, use of antisense RNA, cloning, etc.), biochemical (Western blot analysis, synthesis and degradation of proteins in vitro, GST-pull-down, immunoprecipitation, chromatin precipitation, etc.), biophysical (surface plasmon resonance, mass spectrometry, optical spectrometry, etc.) methods, as well as methods of experimental oncology (tumor growth in experimental animals), immunofluorescence analysis, immunohistochemistry, bioinformatics, and statistics were used.<\/li>\n<\/ul>\n<\/div>\n\n\n<button class=\"accordion-blog\">Grants (2020-2026)<\/button>\n<div class=\"panel-blog\">\n<ul class=\"lab-list\">\n  <li>The role of mitochondrial protein MRPS18-2 and RB in the control of cell stemness and in carcinogenesis, state registration number 0119U103905 (2020-2022)<\/li>\n  <li>Study of molecular mechanisms of inhibition of embryonic tumor growth due to inactivation of MRPS18-2 protein, state registration number 0123U100099 (2023-2026)<\/li>\n  <li>Predictive factors of immune response in oncohematological patients with COVID19 comorbidity, grant of the Ministry of Education and Science within the framework of the HORIZONT 2020 program, state registration number 0123U102171 (2023-2024)<\/li>\n  <li>Activation of signaling cascades for the elimination of transformed B cells in chronic lymphocytic leukemia, grant from the National Fund for the Study of the State Duma, state registration number 0124U003787 (2024-2026)<\/li>\n  <li>Genetic characterization of high-risk human papillomaviruses in Ukraine and Latvia to inform national cervical cancer prevention policy, grant from the Ministry of Education and Science within the framework of joint Ukrainian-Latvian research projects, state registration number 0125U002954 (2024-2026)<\/li>\n<\/ul>\n<\/div>\n\n\n<button class=\"accordion-blog\">Conferences (2021-2025)<\/button>\n<div class=\"panel-blog\">\n<p>RSU Research Week 2021, Riga, Latvia \u00abKnowledge for use in practice\u00bb, 2021<\/p>\n<p>EACR Virtual event, worldwide \u00abBioinformatics in Cancer Programme\u00bb, Nottingen, UK, 2021<\/p>\n<p>MTC day, Karolinska Institutet, Stockholm, Sweden, June 15th, 2022<\/p>\n<p>RSU Research Week 2023, Riga, Latvia \u00abKnowledge for use in practice\u00bb, 2023<\/p>\n<p>E-poster on 18th World Congress of Neurosurgery (WFNS 2023), 4-8 December, Cape Town, South Africa, 2023<\/p>\n<ul class=\"lab-list\">\n  <li>VIRCAN2024 Conference \u00abChronic viral infections and cancer, openings for vaccines and cure\u00bb, Riga, Latvia, 2024<\/li>\n  <li>RSU Research Week 2025, Riga, Latvia \u00abKnowledge for use in practice\u00bb, 26-28 March 2025, Riga, Latvia<\/li>\n  <li>International Scientific Conference &quot;Current Problems of Cell Biology, Microbiology and Biotechnology&quot;, June 12-13, 2025, Lviv<\/li>\n<\/ul>\n<\/div>\n\n\n<button class=\"accordion-blog\">Selected publications (2020-2024)<\/button>\n<div class=\"panel-blog\">\n<ul class=\"lab-list\">\n  <li>Kovalevska L, Kalman S, Sushnova A, Malysheva O, Rozumenko V, Malysheva T, Kashuba E. Differential Expression of MRPS18 Family Genes in Brain Tumor Samples. Exp Oncol. 2025 Feb 20;46(4):368-374. doi: 10.15407\/exp-oncology.2024.04.368.<\/li>\n  <li>Matskova L, Zheng S, Kashuba E, Ernberg I, Aspenstr\u00f6m P. MTSS1: beyond the integration of actin and membrane dynamics. Cell Mol Life Sci. 2024; 81(1): 472. doi: 10.1007\/s00018-024-05511-w.<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b, \u0429\u0435\u0440\u0431\u0456\u043d\u0430 \u0412, \u041a\u0440\u044f\u0447\u043e\u043a \u0406, \u0422\u0438\u0442\u0430\u0440\u0435\u043d\u043a\u043e \u0406, \u041a\u0430\u0448\u0443\u0431\u0430 \u041e. \u0412\u043f\u043b\u0438\u0432 \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u043d\u044f \u043d\u0430 COVID19 \u0442\u0430 \u0432\u0430\u043a\u0446\u0438\u043d\u0430\u0446\u0456\u0457 \u043f\u0440\u043e\u0442\u0438 SARS-COV-2 \u043d\u0430 \u043f\u0435\u0440\u0435\u0431\u0456\u0433 \u043e\u043d\u043a\u043e\u0433\u0435\u043c\u0430\u0442\u043e\u043b\u043e\u0433\u0456\u0447\u043d\u0438\u0445 \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u044c. \u0427\u0430\u0441\u0442\u0438\u043d\u0430 \u0406. \u0413\u0435\u043d\u0435\u0442\u0438\u0447\u043d\u0456 \u0445\u0430\u0440\u0430\u043a\u0442\u0435\u0440\u0438\u0441\u0442\u0438\u043a\u0438 \u0432\u0430\u0440\u0456\u0430\u043d\u0442\u0456\u0432 \u0432\u0456\u0440\u0443\u0441\u0443 SARS-COV-2 \u043f\u0440\u0438 \u0442\u0440\u044c\u043e\u0445 \u0445\u0432\u0438\u043b\u044f\u0445 \u043f\u0430\u043d\u0434\u0435\u043c\u0456\u0457 \u0432 \u0423\u043a\u0440\u0430\u0457\u043d\u0456. \u041e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u044f, 2024 (26)3: 216-221. doi:10.15407\/oncology.2024.03.216<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b, \u0429\u0435\u0440\u0431\u0456\u043d\u0430 \u0412, \u041a\u0440\u044f\u0447\u043e\u043a \u0406, \u0422\u0438\u0442\u0430\u0440\u0435\u043d\u043a\u043e \u0406, \u041a\u0430\u0448\u0443\u0431\u0430 \u041e. \u0412\u043f\u043b\u0438\u0432 \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u043d\u044f \u043d\u0430 COVID19 \u0442\u0430 \u0432\u0430\u043a\u0446\u0438\u043d\u0430\u0446\u0456\u0457 \u043f\u0440\u043e\u0442\u0438 SARS-COV-2 \u043d\u0430 \u043f\u0435\u0440\u0435\u0431\u0456\u0433 \u043e\u043d\u043a\u043e\u0433\u0435\u043c\u0430\u0442\u043e\u043b\u043e\u0433\u0456\u0447\u043d\u0438\u0445 \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u044c. \u0427\u0430\u0441\u0442\u0438\u043d\u0430 \u0406\u0406. \u041e\u0441\u043e\u0431\u043b\u0438\u0432\u043e\u0441\u0442\u0456 \u0435\u043a\u0441\u043f\u0435\u0441\u0456\u0457 \u0440\u0435\u0446\u0435\u043f\u0442\u043e\u0440\u0430 ACE2 \u0443 \u043b\u0456\u043c\u0444\u043e\u0446\u0438\u0442\u0430\u0445 \u0442\u0430 \u0432\u043f\u043b\u0438\u0432 \u0446\u0438\u0442\u043e\u043a\u0456\u043d\u043e\u0432\u043e\u0433\u043e \u0448\u0442\u043e\u0440\u043c\u0443 \u043d\u0430 \u043f\u0435\u0440\u0435\u0431\u0456\u0433 \u043e\u043d\u043a\u043e\u0433\u0435\u043c\u0430\u0442\u043e\u043b\u043e\u0433\u0456\u0447\u043d\u0438\u0445 \u0437\u0430\u0445\u0432\u043e\u0440\u044e\u0432\u0430\u043d\u044c.&lt;w:t&gt;\u041e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u044f, 2024 (26) 4: 301-306.&lt;w:t&gt;doi:10.15407\/oncology.2024.04.301<\/li>\n  <li>Shcherbina V, Kovalevska L, Pedachenko E, Malysheva T, Kashuba E. Comparative analysis of the embryonal brain tumors based on their molecular features. Discovery Medicine. 2023, 35(178): 733-749. doi:10.24976\/Discov. Med.202335178.69<\/li>\n  <li>Zheng S, Wang X, Matskova L, Zhou X, Zhang Z, Kashuba EV, Ernberg I, Aspenstr\u00f6m P. MTSS1 is downregulated in nasopharyngeal carcinoma (NPC) which disrupts adherens junctions leading to enhanced cell migration and invasion. Front Cell Dev Biol. 2023; 11: 1275668. doi: 10.3389\/fcell.2023.1275668.<\/li>\n  <li>Kovalevska L, Kashuba E. Materials of Nobel symposium 175: precision medicine transforms healthcare: a new trajectory for research and innovation September 20-22, 2023, Stockholm, Sweden. Experimental Oncology, 2023, 45(4): 531\u2013534. doi: 10.15407\/exp-oncology.2023.04.531<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b.\u041c., \u041c\u0430\u0442\u0432\u0454\u0454\u0432\u0430 \u0410.\u0421., \u041a\u0430\u0448\u0443\u0431\u0430 \u041e.\u0412. \u0420\u043e\u043b\u044c \u043f\u0440\u043e\u0442\u0435\u0457\u043d\u0430 \u0440\u0435\u0442\u0438\u043d\u043e\u0431\u043b\u0430\u0441\u0442\u043e\u043c\u0438 \u0432 \u0431\u0456\u043e\u043b\u043e\u0433\u0456\u0457 \u0441\u0442\u043e\u0432\u0431\u0443\u0440\u043e\u0432\u0438\u0445 \u043a\u043b\u0456\u0442\u0438\u043d&lt;w:t&gt;\u041e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u044f. 2023, 24 (4): 245-254. doi: 10.15407\/oncology.2023.04.245<\/li>\n  <li>Kovalevska L*, Golenkov O*, Kulahina Y, Callender T, Sizov F, Kashuba E. A. Comparative Study on the Viability of Normal and Cancerous Cells upon Irradiation with a Steady Beam of THz Rays. Life (Basel). 2022, 12(3): 376; doi: 10.3390\/life12030376.<\/li>\n  <li>Govorov I, Attarha S, Kovalevska L, Andersson E, Kashuba E*, Mints M*. Upregulation of PKN1 as a prognosis biomarker for endometrial cancer. Cancer Control. 2022, 29: 10732748221094797; doi: 10.1177\/10732748221094797.<\/li>\n  <li>\u0424\u0435\u0448\u0438\u043d\u0430 \u041c.\u041e., \u041a\u0443\u0447\u0435\u0440\u0435\u043d\u043a\u043e \u0417.\u0413., \u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b.\u041c., \u041a\u0430\u0448\u0443\u0431\u0430 O.\u0412. \u0412\u0438\u0432\u0447\u0435\u043d\u043d\u044f \u0443\u0431\u0456\u043a\u0432\u0456\u0442\u0438\u043d\u0443\u0432\u0430\u043d\u043d\u044f \u0431\u0456\u043b\u043a\u0456\u0432 \u0440\u043e\u0434\u0438\u043d\u0438 MRPS18 in vitro.\u0424\u0430\u043a\u0442\u043e\u0440\u0438 \u0435\u043a\u0441\u043f\u0435\u0440\u0438\u043c\u0435\u043d\u0442\u0430\u043b\u044c\u043d\u043e\u0457 \u0435\u0432\u043e\u043b\u044e\u0446\u0456\u0457 \u043e\u0440\u0433\u0430\u043d\u0456\u0437\u043c\u0456\u0432, 30, 2022, \u0441. 116-121. doi: 10.7124\/FEEO.v30.1471<\/li>\n  <li>Kovalevska L., Malysheva T.A., Kalman S., Rozumenko A.V., Verbova L., Rozumenko V.D., Kashuba E. Expression pattern of MRPS18 family genes in gliomas. Exp. Oncol. 2021, 43 (3): 204-208. doi: 10.32471\/exp-oncology.2312-8852.vol-43-no-3.16461<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b.\u041c., \u041a\u0430\u0448\u0443\u0431\u0430 \u041e.\u0412. \u0424\u0443\u043d\u043a\u0446\u0456\u043e\u043d\u0430\u043b\u044c\u043d\u0456 \u043d\u0430\u0441\u043b\u0456\u0434\u043a\u0438 \u0432\u0437\u0430\u0454\u043c\u043e\u0434\u0456\u0457 \u043f\u0440\u043e\u0442\u0435\u0457\u043d\u0456\u0432 MRPS18-2 \u0442\u0430 RB \u043f\u0440\u0438 \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0456\u0457 \u043a\u043b\u0456\u0442\u0438\u043d\u0438. \u041e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u044f. 2021, 23 (1-2): 1-5. doi: 10.32471\/oncology.2663-7928.t-23-1-2021-g.9384<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b.\u041c., \u0417\u0430\u0434\u0432\u043e\u0440\u043d\u0438\u0439 \u0422.\u0412., \u041c\u0430\u043b\u0438\u0448\u0435\u0432\u0430 \u0422.\u0410., \u041a\u0430\u043b\u044c\u043c\u0430\u043d \u0421.\u0421., \u041b\u0443\u043a\u2019\u044f\u043d\u043e\u0432\u0430 \u041d.\u042e., \u041a\u0430\u0448\u0443\u0431\u0430 \u041e.\u0412. \u041f\u043e\u0440\u0456\u0432\u043d\u044f\u043d\u043d\u044f \u0432\u0456\u0434\u043d\u043e\u0441\u043d\u043e\u0457 \u0435\u043a\u0441\u043f\u0440\u0435\u0441\u0456\u0457 \u0433\u0435\u043d\u0456\u0432 \u0443 \u043f\u0443\u0445\u043b\u0438\u043d\u043d\u0456\u0439 \u0442\u043a\u0430\u043d\u0438\u043d\u0456 \u0442\u0430 \u0443 \u0441\u0438\u0440\u043e\u0432\u0430\u0442\u0446\u0456 \u043a\u0440\u043e\u0432\u0456 \u0445\u0432\u043e\u0440\u0438\u0445 \u043d\u0430 \u0440\u0430\u043a. \u041e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u044f. 2021, 23 (3): 149-153. doi: 10.32471\/oncology.2663-7928.t-23-3-2021-g.9761<\/li>\n  <li>\u041a\u043e\u0432\u0430\u043b\u0435\u0432\u0441\u044c\u043a\u0430 \u041b.\u041c., \u041a\u0430\u0448\u0443\u0431\u0430 \u041e.\u0412. \u0420\u043e\u043b\u044c \u043f\u0440\u043e\u0442\u0435\u0457\u043d\u0456\u0432 \u0440\u043e\u0434\u0438\u043d\u0438 MRPS18 \u0443 \u0437\u043b\u043e\u044f\u043a\u0456\u0441\u043d\u0456\u0439 \u0442\u0440\u0430\u043d\u0441\u0444\u043e\u0440\u043c\u0430\u0446\u0456\u0457 \u043a\u043b\u0456\u0442\u0438\u043d\u0438. \u0428\u043b\u044f\u0445\u0438 \u0442\u0430 \u043f\u0435\u0440\u0441\u043f\u0435\u043a\u0442\u0438\u0432\u0438 \u0440\u043e\u0437\u0432\u0438\u0442\u043a\u0443 \u0435\u043a\u0441\u043f\u0435\u0440\u0438\u043c\u0435\u043d\u0442\u0430\u043b\u044c\u043d\u043e\u0457 \u043e\u043d\u043a\u043e\u043b\u043e\u0433\u0456\u0457 \u0432 \u0423\u043a\u0440\u0430\u0457\u043d\u0456: \u0432\u0438\u043f.2. \u2013 \u041a.: \u0414\u0406\u0410, 2021.\u2013 \u0421. 77-90. ISBN 978-617-7785-41-4<\/li>\n  <li>Rasa-Dzelzkaleja S, Gravelsina S, Chapenko S, Krukle ZN, Svirskis S, Suna N, Kashuba E, Karelis G, Murovska M. Persistent Roseoloviruses Infection in Adult Patients with Epilepsy. Brain Sci. 2020; 10(5): 287. doi: 10.3390\/brainsci10050287.<\/li>\n  <li>Mushtaq M, Kovalevska L, Darekar S, Abramsson A, Zetterberg H, Kashuba V, Klein G, Arsenian-Henriksson M, Kashuba E. Cell stemness is maintained upon concurrent expression of RB and the mitochondrial ribosomal protein S18-2. PNAS, June 22, 2020. doi: 10.1073\/pnas.1922535117.<\/li>\n<\/ul>\n<\/div>\n\n\n<button class=\"accordion-blog\">Collaborations (2021-2025)<\/button>\n<div class=\"panel-blog\">\n<p>1. State Institution &quot;A.P. Romodanov Institute of Neurosurgery&quot; of the National Academy of Medical Sciences of Ukraine. Study of processes of control of cell stemness in carcinogenesis. Contract 2020-2023 (with automatic extension).<\/p>\n<p>2. V.E. Lashkarev Institute of Semiconductor Physics of the National Academy of Sciences of Ukraine. Study of fundamental mechanisms of cell transformation and the effect of low-frequency radiation on cell survival. Contract 2021-2024 (with automatic extension).<\/p>\n<p>3. Karolinska Institutet, Stockholm, Sweden. Implementation of joint research to clarify the significance of signaling networks in malignant cell transformation. Cooperation Agreement dated 04\/25\/2013, unlimited term.<\/p>\n<p>4. Riga Stradi\u0146\u0161 University, Riga, Latvia. Conducting joint research aimed at studying malignant transformation and signaling pathways regulating it. Cooperation agreement dated 02\/05\/2016, unlimited term.<\/p>\n<\/div>\n\n\n<button class=\"accordion-blog\">People \u2013 (2024)<\/button>\n<div class=\"panel-blog\">\n<p>1. Elena Kashuba, PhD, Dr Hab, Professor, Head of Lab.<\/p>\n<p>2. Larysa Kovalevska, PhD, docent, Senior Researcher, studying for a Dr Hab since 2023<\/p>\n<p>3. Alina Matveeva, PhD, Junior Researcher<\/p>\n<p>4. Serhiy Kalman, Master of Science, Engineer, studying for a postgraduate degree since 2023<\/p>\n<p>5. Elizaveta Kulagina, Master of Science, Engineer<\/p>\n<p>6. Anna Sushnyova, Bachelor of Science, Engineer<\/p>\n<p>7. Ivan Eremenko, Bachelor of Science, Engineer<\/p>\n<p>8. Luidmila Voznyuk, Technician<\/p>\n<p><strong>Our students (2021-2025)<\/strong><\/p>\n<p><strong>2021<\/strong><\/p>\n<p>1. Anastasia Kolesnikova, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Master\u2019s qualification work \u201cExpression pattern of MRPS18 (1-3) family genes in blood serum and tumor tissues in prostate cancer\u201d, supervisor \u2013 L. Kovalevska.<\/p>\n<p>2. Sergey Kalman, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Bachelor\u2019s qualification work \u201cThe role of mitochondrial protein MRPS18-2 in carcinogenesis\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p>3. Zinaida Kucherenko, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Course work \u201cStudy of stability of MRPS18-2 protein in vitro and in mammalian cell cultures\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p><strong>2022<\/strong><\/p>\n<p>1. Zinaida Kucherenko, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Bachelor\u2019s qualification work \u201cUbiquitination of MRPS18-2 protein in in vitro systems\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p>2. Elizaveta Kulagina, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Course work \u201cApplication of genetic engineering constructs of the MRPS18-2 protein gene of different domain structure for studying its ubiquitination\u201d, supervisor \u2013 L. Kovalevska.<\/p>\n<p>3. Serhiy Kalman, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Course work \u201cThe role of mitochondrial protein MRPS18-2 in embryonic tumors\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p><strong>2023<\/strong><\/p>\n<p>1. Dmytro Savin, Taras Shevchenko Kyiv National University, National Center \u201cInstitute of Biology and Medicine\u201d. Bachelor\u2019s qualification work \u201cThe influence of MRPS18-2 protein overexpression on the rate of proliferation, clonal formation and directed differentiation in vitro of the retinoblastoma cell line WERI-RB-27\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p>2. Elizaveta Kulagina, Taras Shevchenko Kyiv National University, National Center \u201cInstitute of Biology and Medicine\u201d. Bachelor\u2019s qualification work \u201cThe influence of MRPS18-2 protein overexpression on the rate of proliferation, clonal formation and directed differentiation in vitro of the melanoma cell line A375\u201d, supervisor \u2013 L. Kovalevska.<\/p>\n<p>3. Serhiy Kalman, Taras Shevchenko Kyiv National University, National Center \u201cInstitute of Biology and Medicine\u201d. Master&#x27;s qualification work &quot;The role of mitochondrial protein MRPS18-2 in the growth of embryonic tumors&quot;, supervisor &#8212; E. Kashuba.<\/p>\n<p>4. Kateryna Shaduro, Taras Shevchenko National University of Kyiv, National Center &quot;Institute of Biology and Medicine&quot;. Bachelor&#x27;s qualification work &quot;The influence of genetic engineering constructs encoding proteins MRPS18-2 and RB on the survival of WERI cells during THz irradiation&quot;, supervisor &#8212; L. Kovalevska<\/p>\n<p><strong>2024<\/strong><\/p>\n<p>1. Dmytro Savin, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Coursework, 1st year of master\u2019s degree \u201cThe influence of MRPS18-2 protein overexpression on the rate of proliferation, clonal formation and directed differentiation in vitro of the retinoblastoma cell line WERI-RB-27\u201d, supervisor \u2013 E. Kashuba.<\/p>\n<p>2. Elizaveta Kulagina, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Coursework, 1st year of master\u2019s degree \u201cThe influence of MRPS18-2 protein overexpression on the rate of proliferation, clonal formation and directed differentiation in vitro of the melanoma cell line A375\u201d, supervisor \u2013 L. Kovalevska.<\/p>\n<p>3. Serhiy Mosnytskyi, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Coursework, 3rd year of bachelor&#x27;s degree &quot;Study of the expression pattern of KLF4 and KLF6 genes in embryonic brain tumors&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p>4. Hanna Monchakivska, Kyiv National University named after Taras Shevchenko, NSC &quot;Institute of Biology and Medicine&quot;. Bachelor&#x27;s qualification work &quot;Study of mechanisms of inactivation of TGFB signaling in CLL cells&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p>5. Ivan Eremenko, Kyiv National University named after Taras Shevchenko, NSC &quot;Institute of Biology and Medicine&quot;. Coursework, 3rd year of bachelor&#x27;s degree &quot;The effect of overexpression of MRPS18-2 protein on the proliferation rate and directed differentiation in vitro of the Kelly neuroblastoma cell line&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p>6. Anna Sushnyova, Kyiv National University named after Taras Shevchenko T. Shevchenko, NSC &quot;Institute of Biology and Medicine&quot;. Bachelor&#x27;s qualification work &quot;Study of the expression pattern of MRPS18 family genes in embryonic brain tumors&quot;, supervisor &#8212; E. Kashuba.<\/p>\n<p><strong>2025<\/strong><\/p>\n<p>1. Elizaveta Kulagina, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Master\u2019s qualification work \u201cFunctional changes in melanoma cells upon introduction of genetically engineered constructs of the MRPS18 family\u201d, supervisor \u2013 L. Kovalevska.<\/p>\n<p>2. Vladyslav Khreshchenyuk, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Master\u2019s qualification work \u201cStability of mRNA and kinetics of its decay for MRPS18 family genes in breast cancer cells\u201d, supervisor \u2013 O. Kashuba.<\/p>\n<p>3. Anna Sushnyova, Taras Shevchenko National University of Kyiv, NSC \u201cInstitute of Biology and Medicine\u201d. Coursework, 1st year of master&#x27;s degree &quot;Study of the expression pattern of MRPS18 family genes in embryonic brain tumors&quot;, supervisor &#8212; O. Kashuba.<\/p>\n<p>4. Hanna Monchakivska, Kyiv National University named after Taras Shevchenko, NSC &quot;Institute of Biology and Medicine&quot;. Coursework, 1st year of master&#x27;s degree &quot;Study of mechanisms of cytokine signaling pathways in CLL&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p>5. Ivan Eremenko, Kyiv National University named after Taras Shevchenko, NSC &quot;Institute of Biology and Medicine&quot;. Bachelor&#x27;s qualification work &quot;The effect of MRPS18-2 protein overexpression on the biological properties of Kelly neuroblastoma cell line&quot;, supervisor &#8212; O. Kashuba.<\/p>\n<p>6. Serhiy Mosnytskyi, Kyiv National University named after Taras Shevchenko T.Shevchenko National University of Kyiv, NSC &quot;Institute of Biology and Medicine&quot;. Bachelor&#x27;s qualification work &quot;Features of expression of KLF4 and KLF6 genes and their association with MRPS18-2 in brain tumors&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p>7. Sofia Lytvyn, T.Shevchenko National University of Kyiv, NSC &quot;Institute of Biology and Medicine&quot;. Course work &quot;Features of expression of transcription factors specific for B-cells in CLL&quot;, supervisor &#8212; L. Kovalevska.<\/p>\n<p><strong>Advanced training (2021-2025)<\/strong><\/p>\n<p>During the period 2020-2024 E. Kashuba and L. Kovalevska interned at the Karolinska Institutet, Stockholm, Sweden (2 months in total each year).<\/p>\n<p>A. Sushnyova interned at the Ecole Polytechnique federale de Lausanne, Lausanne, Switzerland, as part of the summer research program (2 months, 2025)<\/p>\n<p>A brief historical background about the department (laboratory)<\/p>\n<p>After the structural reorganization of the R.E. Kavetsky IEPOR of NAS of Ukraine in January 2020, a laboratory of molecular mechanisms of cell transformation was created on the basis of the disbanded Department of Molecular and Cellular Pathobiology, headed by Corresponding Member of the NAS of Ukraine, Professor Svitlana Pavlivna Sydorenko.<\/p>\n<\/div>\n\n","protected":false},"excerpt":{"rendered":"<p>Head Elena Kashuba Dr Sci (Biol.), Professor +38 (044) 259-01-88 Kashuba@nas.gov.ua, lenakash@yahoo.com Priority areas of research Our laboratory&#x27;s research is carried out within the framework of the Institute&#x27;s scientific directions aimed at identifying markers of malignant cell transformation and signs of stemness in tumor cells. The obtained results will be the background for development of [&hellip;]<\/p>\n","protected":false},"author":1,"featured_media":0,"parent":35,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-585","page","type-page","status-publish","hentry"],"_links":{"self":[{"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/pages\/585","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/users\/1"}],"replies":[{"embeddable":true,"href":"https:\/\/en.iepor.site\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=585"}],"version-history":[{"count":5,"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/pages\/585\/revisions"}],"predecessor-version":[{"id":601,"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/pages\/585\/revisions\/601"}],"up":[{"embeddable":true,"href":"https:\/\/en.iepor.site\/index.php?rest_route=\/wp\/v2\/pages\/35"}],"wp:attachment":[{"href":"https:\/\/en.iepor.site\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=585"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}